The Wireless Telecommunications Industry

The wireless Telecommunications Industry Wireless communication is one sector of business that has undergone phenomenal growth over the past few years. The number of individuals using mobile devices like cell phones, I phones, laptop and palm to computers has drastically increased even in the developing and third world countries. This growth in market has attracted a lot of attention in this sector attracting new entrants to compete with the traditional communication giants.Advertising We will write a custom term paper sample on The Wireless Telecommunications Industry specifically for you for only $16.05 $11/page Learn More Monopolies are described in economics as the existence of a firm or corporation that has so much control over the market that they have a significant influence on whether a new entrant in the market will succeed or not and if they do enter, on what terms (Heywood and Guangliang, 2009). Monopolies are generally discouraged in most eco nomies with laws put in place to regulate it. Certain monopolistic behaviour can sometimes play to the disadvantage of the smaller new entrants thus the need of regulation to avoid unfair competition. Oligopolies on the other hand are markets whereby there are just a few product or service providers. This is normally characterised by stiff competition and marketing strategies that always have the competitor’s reaction in mind. Oligopolies have sometimes been perceived better than monopolies because the end consumer then has a sense of choice and the cost of products and services are self regulated (Heywood and Guangliang, 2009). The wireless communication industry is probably one of the best examples of oligopolistic markets. For many years there have been just but a handful of wireless communication firms dominating the market. The main players have been Nokia, Samsung, Sony Ericsson and LG; but with growing markets, new entrants have been attracted into the market. The i ph one by apple is probably the best example of a new entrant. Apple focussed on great innovation to take mobile communication to a whole new level. They came up with the I phone that allows the users to get their e mails directly on their phone just like text messages while supporting all the other conventional phone features. China has also come into the market with phones similar to the ones made by the original players. All these combined have put pressure on the traditional top players both in terms of pricing and innovation. The china phones are a lot cheaper but perform all the functions as the original phones plus extras like TV reception. I do not however think that the competition can push out some players because despite the cheap prices, the cheap phones have some downsides like shorter lives and poor batteries. The traditional leaders are therefore almost guaranteed to keep the upper class markets.Advertising Looking for term paper on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More The stock prices of the big firms are not likely to be affected because if the firms maintain top quality products then their reputations will remain protected. Reseller phones now available in the market are likely to work against the cheaper Chinese phones rather than the large firms because of the prices. The big firms in the market are only likely to change their strategies in separating their markets. This they can easily achieve by dedicating a department within their firm to cater for the lower end market by providing good quality phones but at affordable prices. This would take care of the competition from china. They would also need to invest in innovation to counter competition from the ever emerging new technologies. They would also need to diversify their products to include other services. The graph below shows how these services were distributed in Japan (home of the giants in telecommunications in dustry) for the year 2009. Products Services Figure 1. IBISWorld (2009) After all is said and done, it is the end consumer that is bound to benefit from all this competition and innovativeness because they will get quality products at affordable prices. References Heywood, J., Ye, G. (2009). Delegation in a mixed oligopoly: the case of multiple private firms. Managerial and Decision Economics, 30(2),  71- 82.Advertising We will write a custom term paper sample on The Wireless Telecommunications Industry specifically for you for only $16.05 $11/page Learn More IBISWorld .(2009). Products and services[pie chart]. Retrieved from IBISWorld, Wireless Telecommunications Carriers https://www.ibisworld.com/industry-trends/market-research-reports/information/broadcasting-telecommunications/wireless-telecommunications-carriers.html This term paper on The Wireless Telecommunications Industry was written and submitted by user Braelynn Mccarthy to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Antrax essays

Antrax essays Antrax is a rough infectious disease caused by Bacillus anthracis, a positive gram bacteria, aerobe formed from endospores, encapsulated, that can be half ordinary cultivated. The vegetative cell is large (1-8 microns in longitude, 1-1.5 microns wide). The size of a spore is approximately 1 micron. Spores grow rapidly in all cultivated ordinary labs at 37degrees celcius. Its cell morphology and colonial allows its identification by an experienced microbiologist, even though few microbiologists outside of the vetinary community have seen antrax colonies more than in text books. The three virulence factores are: the edema toxin, the deadly toxin and a capsular antigenic. Etymology: Anthracis originated from the Greek word for carbon, anthrakis, because the disease causes black superficial injuries like carbon. The toxic properties of B. anthracis were not recognized until 1954. Previously, due to the tremendous number of observed bacteria in infected animal blood (>109 bacteria/ ml), it was supposed that death was due to the capillary obstruction, But experimentaly it was demonstrated that only approximately 3 million cells/ml are necessary to cause the animals death. Besides, the animals plasmic infection cells contain a toxic that causes antrax symptoms when it is injected in normal rabbits. From these observations it is concluded that exotoxin plays an important role in the pathogeny of antrax. Cepas: There are two even varient colonial(s) a rough(r) that are related with the ability to form a capsule. The R varients are relativaly avirulent. The capsule is not toxic, it acts as protectionj against phagocytosis and it plays its most important role during the establishment of the disease, and a less significant role in the last phase of the disease that is measured by antrax toxin. An antrax toxin component has a deadly action form that at this time is not discovered. Death is apparantly due to th ...

Project management Research Paper Example | Topics and Well Written Essays - 3500 words

Project management - Research Paper Example A project is not a routine task, it is a certain set of functions designed to achieve a particular goal. Sometimes the project team involves people who usually do not work together; they are acquired from different departments, different organizations & different localities. In simple terms, Project Management involves a temporary group activity. Project Management is related to the application of knowledge, techniques & skills to complete a project efficiently & effectively. It is a strategic decision by the organization to enhance their competencies by tying the project outputs to the organization’s objectives. Previously project management referred to the collection of project data & metrics for evaluation & then accordingly making changes to increase efficiency & productivity. For the past decades, this area of management have become quite popular within the corporate world because of the following reasons- (ds.psu.edu 2) The average salary of a project manager is quite hi gh in comparison to the managers of other domain. Every year, a huge amount is spent for the purpose of accomplishing various projects. Lately, project management has been included as a subject of study in various educational institutes. It acts as a control mechanism for various projects. Project management helps in controlling the human capital, budget, quality, resources, time frames & communication. Project Life Cycle Projects always have a starting date & an ending date which means just like products, projects also have a definite life cycle that starts with the starting date & ends with the ending date. The diagram shows the different phases of a project life cycle- (weill.cornell.edu 1) Here we are given a particular business case where we have to modify the present structure of the University into a Wi-Fi enabled campus. We need to replace all computers & introduce iMacs for the classroom. Also we are required to complete the job within the month of June as the research woul d start from July onwards. We need several staffs for this purpose. Selection of the appropriate option for the given business case The appropriate solution that has been selected for the business case is requesting financial aid from the government as it is a reliable source of funds. On the basis of this option we are going to discuss further about this case with the help of the A3 tool. The context of Toyota can be considered while exploring the concept of A3. The managers of the company implement a tool called A3 that is named keeping in mind the internationally accepted paper size.A3 consists of a series of boxes arranged in a template. The boxes include the operations in the following order- 1. To establish the context of the business & the importance of a specific issue 2. To define the present status of the problem 3. To identify the desired outputs 4. To analyze the circumstances to ascertain causality 5. To offer countermeasures 6. To establish an action plan 7. To chalk o ut the follow-up process This method helps the managers to train others regarding the analysis of the root-cause & channel them towards scientific thinking. This is a tool for constant improvement & learning. We are required to use one management tool for the current business case & we would consider applying the A3 framework.

Are Rising U.S. Tensions with Russia and China Signs of a Return to Research Paper

Are Rising U.S. Tensions with Russia and China Signs of a Return to the Cold War - Research Paper Example 4. The missile defense system has caused Russia to deploy its ballistic missiles across Europe. 5. China and Iran have a close eye on US movements in Afghanistan, and its trembling relations with Pakistan. There are negligible or no chances of a second cold war among US, Russia and China because: 1. The three nations are matured and developed to make a mistake they made long ago. 2. It is not in the strategic interest of the three nations that such a war takes place. 3. There is much at stake, and a lot to lose if the war eventually breaks out. 4. US has its hands full with Afghanistan and Pakistan, it will risk going to war with a super power which can cause irreparable damage. 5. Iran is of significant concern to US at the moment. Americans will not risk Iran becoming a nuclear power for notorious reasons. Let us consider the chances of another cold war erupting among the Three Titans. The first cold war started in 1945 and went on till 1991, with the eventual defeat of the Soviet Union. The named cold war comes from the ability of nations to be in a state of war without direct military involvement. Military action is to be avoided because of the fact that it would bring a nuclear catastrophe to the nations’ door step. ... War.1 The question in one’s particular mind would arise that if there is no military involvement, then how is it possible to be in a war situation? These battles are fought through military coalitions, strategic conventional force deployment, and nuclear arms race. The rivalry is also seen at the level of sports and technology. The most famous technological competition between US and Russia is the Space Race. Military alliances such as the US led NATO and the Soviet Union led Warsaw Pact agreements were enforced to display military might.2 Considering the current situation between the Titans, we can asses that an environment is being built towards such a catastrophe. China seems to be very upset with the US movements in Afghanistan and its influence in the political mainstream of Pakistan. On the other hand, Russians are strongly condemning the US movements on the European soil. Its move to deploy Missile shields have irritated the Russians to an extent that led to a Georgian pounding some time back. Georgia is an ally of the US, but at the time when Russia was all out thrashing it, the Americans could only sit and watch the show because any US move would have been at the cost of war with Russia. The story of conflict does not end with Europe or Pakistan, Missile Shield or the US movements in Afghanistan. The three nations are of very different opinion on the matter of Iran. The Islamic republic of Iran is accused by the US of developing nuclear weapons or the WMDs. Iran suggests that its nuclear program is for civilian reasons and it has the right to use nuclear technology for civilian reasons. The US has tightened its sanctions on Iran and is threatening it to go to any limit possible to stop it from developing nuclear weapons. The extent could be by bombing its

Designing and Integrating Information System

Designing and Integrating Information System 1. INTRODUCTION As computer technology changes at fast pace, many businesses sectors also upgrade their computer system constantly in order to stay competitive. For such, the function has become indispensable asset of the companies that process enormous amount of data and complex transactions. Payroll is an example of a complex transaction a company has. Its preparation is crucial since it involves accounts and requires confidentiality. The use of an integrated information system will facilitate the accomplishment of jobs easier, faster and more efficiently. According to Kanchov (2006), application program is the bridge between the users and the database, where data are stored. Thus, a well-developed application program and database ensure the reliability, flexibility, and functionality of an information system. Information technology use aims at increasing the efficiency and reliability of management and monitoring of records. Steven M. Bragg (2006) says that in payroll system the timekeeping function is coming under increasing attack, as cost accountants realize that the cost of administering a detailed timekeeping system is exceeding the value of the result information. This issue can be resolved either by reducing the level of timekeeping effort until the effort expanded equals the utility of the resulting information (which may result in the complete elimination of the timekeeping function) or by more fully automating the timekeeping and payroll functions, so that the cost of the system administration is reduced to the point where it is once again a cost-effective means of tracking labor activities. According to Chowdury (2008), Inventory System is known to be used by many financial institutions. PIM (Perpetual Inventory Method) system maintains an up-to-date record of accurate level of goods at hands by ensuring that stocks are accounted for at all times. The process includes details of all recording purchase and sales receipts and issues and running balances of all stocks. In line with this, the researchers proposed for Balaod Mindanaw Incorporated a LAN Base system for a fully secured and reliable data, easy maintenance, and quicker data retrieval. Balaod Mindanaw Incorporated has decided to automate its transactions. BALAOD-Mindanaw traces its beginnings to Balay Mindanaw Foundation, Inc. (BMFI) legal program as implemented by the LARGE (Legal Assistance for Agrarian Reform, Governance and Empowerment) Unit. BALAOD Mindanaw is a non-stock, non-profit organization that aims to help in the advancement of the legal and justice issues of different marginalized sectors and communities in Mindanao in the context of active peoples participation in governance. BALAOD sets forth in following organizational goals to promote paralegal formation and provide other legal services to marginalized sectors and communities in Mindanao; to conduct capability-building interventions on local legislation and dispute resolution; to provide a venue for networking and alter native legal assistance for law practitioners, law schools and law students in Mindanao; and to facilitate the creation of a favorable policy environment responsive to the needs of marginalized sectors and communities in Mindanao. 2. FRAMEWORK This study designed and developed an integrated information system to facilitate better operations of the organization. The researchers used the Practical Approach to Payroll Administration and Shared Services Approach. Practical Approach will create an awareness of statutory laws (eg. Employment Act, CPF Act, SDF Act, Income Taxt Act, and others) and give the overview of the components of a computerized payroll system. Also the approach will help develop an understanding of compensation components and the latest trends affecting payroll administration ( Subbu and Daofen, 2006). Shared Services Approach is an operational philosophy that involves centralizing administrative functions that were once performed in divisions or locations. Services that can be shared among the various business units of a company include finance, purchasing, inventory, and payroll (Sherman, 2008). Figure 1 illustrates the context diagram of developed Integrated Information System for Balaod Mindanaw, Inc. It shows the system relationship for the four entities: the Finance Officer, Employee, Librarian, and the Supply Officer. The system will automatically calculate the number of work hours of every employee and the deduction for every employees absence and under time. The system will send the information to other entities, applying the shared service approach by which information like employee information, books available in the library, and budget and inventory of every department are circulated. 3. Objective of the Study 3.1 General Objective of the study This study aimed at designing and developing an Integrated Information System for Balaod Mindanaw, Inc. The system is intended to minimize the effort of the user in the processing of payroll slip, payroll form, library slip, library form and in the monitoring of supplies. Also, the system is intended to create a well-arranged database to implement strong data security system. 3.2 Specific Objectives of the study The study sought to accomplish the following specific objectives: 3.2.1. To access the present payroll system of Balaod Mindanaw, Inc. 3.2.2. To profile the respondents in terms of age, gender, civil status, birth date, birth place, province, home address, current home address, family information, and education. 3.2.3. To identify the underlying problems of the present system involving the processes on payroll, library, inventory and the monitoring of supplies. 3.2.4. To determine the accuracy and effectiveness of the present system and the possible benefits of the proposed information system. 4. Statement of the Problem The study specifically sought to address the following problems of Balaod Mindanaw Inc: 4.1. The HR uses only papers to record employee information. Such system slows down the retrieval of data. 4.2. The file can easily be damaged and lost. 4.3. Double checking the consistency of supply records is time-consuming. 4.4. Financial computations are time-consuming and prone to mathematical errors that may cause financial or legal trouble. 5. Scope and Limitation The proposed system covers the process of budget monitoring for every department, requisition of equipment and books in the library, inventory of supplies, and monitoring of expenses of every department. The proposed payroll system covers the keeping of Daily Time Record for computing the exact wages of each employee. The system can do the following: 5.1. Compute monthly net income, deductions, and loans such as SSS, Philhealth, COLA, Emergency loans, PAG IBIG, and withholding tax. 5.2. Calculate budget allocation plus expenses of every department 5.3. Add book borrows records 5.4. Add employee information 5.5. Add employees login and logout records 6. Significance of the Study The proposed system will benefit the following entities: The Manager. The system will provide the manager with reliable and accurate reports concerning the companys budget and equipment use. The Supply Officer. The system will help the supply officer conduct an inventory of the supplies to generate reliable reports for referencing. The Finance Department. The system will help the finance department monitor all the expenses of every department and conduct liquidation anytime. Also, the department can process the payroll faster and accurately. The Company. The system will give the company the advantage over other companies that still use the traditional way of running the operations. Also, it will help the company increase its performance to satisfy its clients. The Future Researchers. The findings of the study will serve as basis for developing related system for other companies or establishments. 7. Output The output of the study is the Integrated Information System for Balaod Mindanaw, Incorporated. A complete Program Structure, Database Specifications and Design, and System User Manual go with the software. All program source codes are also fully documented and compiled. 8. Research Methodology The researchers used the simplified water fall model, which illustrates the sequential software development process in which progress is seen as flowing steadily downwards through the phases. Water fall development has distinct goals for each phase development from conceptualization through designing, implementation, testing, installation, troubleshooting, deployment of the system, and maintenance. 8.1. The Requirement Gathering and Analysis. The researchers surveyed literature and interviewed the user about the companys present system so that the system limitations could be identified and addressed 8.2. System Design.A system with applications addressing the present systems limitations was designed and developed. 8.3. Implementation. The developed system was applied given the permission of the company. 8.4. Testing.The developed system was subjected to interface testing, functionality testing, and front-end and back-end integration testing. 8.5. Deployment of the System.After a series of testing, the system was deployed to the working environment. 8.6. Maintenance. Lastly, it is very likely for the developed system to encounter system errors and unexpected bugs. Thus, the researchers will diagnose and resolve unknown issues. Further, the researchers will consider further enhancement of the database system. 8.1 CURRENT SYSTEM Narrative Description of the Current System First, every department submits a projected expense to the finance department or a proposed budget for the entire year, and then the finance department presents the projected expense of every department to the manager for approval. If the budget is approved, the finance department grants the approved budget to the department to be utilized within one year. If the budget is not approved, the finance department returns the projected expense to the department for adjustment or modification. Second, every department requests for supply to the supply officer. The supply officer asks the finance department for the budget. Then the budget for supply is submitted to the manager for approval. If the budget is approved, then the finance department gives the budget to the supply officer for the purchase of the requested supply. Then the purchased supply is given to the requesting department. 8.2 PROPOSED SYSTEM Narrative Description of the Proposed System The employee registers to indicate login and logout. The registration serves as reference for payroll. Every department sends its budget request through the system. The finance can view the budget request. The finance then presents the budget to the manager for approval. Once the requested budget is approved, the finance notifies the department as to the approval of the budget. The manager can view all transactions. For the supply requisition, the department sends the request to the supply officer. Then the supply officer forwards the request to the finance officer for the budget. Once the budget for the supply is approved by the finance officer, the supply officer canvasses and purchases the requested supply. The supply will then be delivered to the requesting department. The supply officer monitors the deliveries and declares the status of materials, and decides replacement of them. The finance department can add employees records and accounts and view the expenses of the entire de partment. The librarian handles all the book and journals. All book records are kept by a library staff. If the employee borrows a book, the librarian records the details of the book and the borrowers name. If the book is lost by the borrower, the librarian submits a note about the lost to the HR Department. 9.2 DATABASE STRUCTURE The system uses one database, which is the IISBalaodMindanao. It contains many tables and stores information such as employees record, daily time record, basic salary. And also, the system monitors the status of books in the library and other essential data. 9.3 Data Dictionary EmployeesProfile FamilyInfo WorkExperience WorkDescription Organization ImportantSeminar EducationalProfile Skills Talents Interest Logout Login Books BookDetails BookBarrowed DATA FIELD TYPE DATA TYPE

Dagoberto Gilb :: Essays Papers

Dagoberto Gilb Dagoberto Gilb was born in Los Angeles in 1950. A mix of gritty humor, mundane terror, and economic misfortune distinguishes his short stories. His life has been neither easy nor subdued, and these influences are reflected in his writing style and choice of subject matter. The short story entitled â€Å"Love in L.A.,† by Dagoberto Gilb, shows how one can see many reasons in seeing irony and even satire by the story’s title and how all is stories combine in someway. Dagoberto Gilb’s childhood was spent running wild in a bad part of Los Angeles. At the age of eighteen, Gilb decided to attend college, obtaining degrees in Philosophy and Religious studies. It was during this period that he began to keep personal notebooks. Following completions of a master’s degree, he became a journeyman carpenter from 1976 – 1991, which provided the flexibility to devote large blocks of time to writing. He is married to a woman named Rebeca, and has two children named Antonio and Ricardo. Gilb worked in the Department of English at the University of Texas in 1988, the University of Arizona in 1992, and the University of Wyoming in 1994 (â€Å"Dagoberto Gilb†) Literary magazines were not remotely interested in publishing Gilb’s stories, which focus primarily on the professional and personal struggles of working-class Mexican Americans. But his unapologetic stories about working-class Mexican Americans have made him a voice of his people (Reid130). Gilb’s short stories are set vividly in cites of the desert Southwest and usually feature a Hispanic protagonist who is good-hearted but often irresponsible and is forever one pink slip or automotive breakdown away from disaster (Reid130). Gilb was persuaded to submit some of the 750 pages of his unpublished stories to the National Endowment of the Arts, and, when he won a grant, it enabled him to take time to put together a collection of his work. That was The Magic of Blood, which was published in 1993 and won him a PEN Hemingway Award. The following year, recognition abounded in the form of prestigious literary awards and a flood of critical attention for The Magic of Blood (â€Å"The Magic of Blood†63-74) Gilb has earned many awards including the Institute of Letters Award for best book of fiction and best short story in 1993. In El Paso, he gave up on New York publishing and sold his story collection to the University of New Mexico Press. Dagoberto Gilb :: Essays Papers Dagoberto Gilb Dagoberto Gilb was born in Los Angeles in 1950. A mix of gritty humor, mundane terror, and economic misfortune distinguishes his short stories. His life has been neither easy nor subdued, and these influences are reflected in his writing style and choice of subject matter. The short story entitled â€Å"Love in L.A.,† by Dagoberto Gilb, shows how one can see many reasons in seeing irony and even satire by the story’s title and how all is stories combine in someway. Dagoberto Gilb’s childhood was spent running wild in a bad part of Los Angeles. At the age of eighteen, Gilb decided to attend college, obtaining degrees in Philosophy and Religious studies. It was during this period that he began to keep personal notebooks. Following completions of a master’s degree, he became a journeyman carpenter from 1976 – 1991, which provided the flexibility to devote large blocks of time to writing. He is married to a woman named Rebeca, and has two children named Antonio and Ricardo. Gilb worked in the Department of English at the University of Texas in 1988, the University of Arizona in 1992, and the University of Wyoming in 1994 (â€Å"Dagoberto Gilb†) Literary magazines were not remotely interested in publishing Gilb’s stories, which focus primarily on the professional and personal struggles of working-class Mexican Americans. But his unapologetic stories about working-class Mexican Americans have made him a voice of his people (Reid130). Gilb’s short stories are set vividly in cites of the desert Southwest and usually feature a Hispanic protagonist who is good-hearted but often irresponsible and is forever one pink slip or automotive breakdown away from disaster (Reid130). Gilb was persuaded to submit some of the 750 pages of his unpublished stories to the National Endowment of the Arts, and, when he won a grant, it enabled him to take time to put together a collection of his work. That was The Magic of Blood, which was published in 1993 and won him a PEN Hemingway Award. The following year, recognition abounded in the form of prestigious literary awards and a flood of critical attention for The Magic of Blood (â€Å"The Magic of Blood†63-74) Gilb has earned many awards including the Institute of Letters Award for best book of fiction and best short story in 1993. In El Paso, he gave up on New York publishing and sold his story collection to the University of New Mexico Press.

Membrane Permeability

The Neurophysiology of Nerve Impulses and Effects of Inhibitory Chemicals on their Action Potentials Aferdita Sabani Biol 2401. C5L Dr. Endley March 20, 2013 Introduction Cell structure and function can be defined in many aspects but one the most important characteristic is that it is enclosed within a cell membrane called a plasma membrane. The plasma membrane is by-layer composed of lipids and embedded proteins. This membrane is semi-permeable due to its hydrophobic and hydrophilic regions.At the boundary of every cell the plasma membrane functions as a selective barrier that allows nutrients to be brought in and/or removed from inside the cell. The cells permeability and transport mechanisms allow for this occurrence and it is vital for a functional and healthy cell. Transport through the plasma membrane occurs in two basic ways: passive and active processes. The passive transport process is driven by the concentration or pressure differences between the interior and exterior envi ronment of the cell.According to Kenyan college biology department, â€Å"Simple diffusion is when a small non-polar molecule passes through a lipid bilayer. It is classified as a means of passive transport. In simple diffusion, a hydrophobic molecule can move into the hydrophobic region of the membrane without getting rejected†. Particles diffuse passively through small pores within the plasma membrane and they also move from an environment of high concentration towards an environment with lower concentration. Osmosis is a type of diffusion when it comes to water transport.Both diffusion and osmoses move substances down their concentration gradient. Facilitated diffusion is also passive transport, but does not involve the simple movement through pores and lipid dissolving. In this case a carrier protein in the membrane is introduced to facilitate the transport of substances down their concentration gradient. Active transport is not passive because energy in the form of cellu lar ATP is required to drive the substances across the membrane, therefor the cell must spend some f its energy to get through or move against the concentration gradient. In one type of active transport the substance gets across the membrane by forming a substrate –enzyme complex where the substance is picked up by a carrier protein and are then able to move into cell. This combination is lipid and large so energy is needed to defy opposing forces. According to Pearson/biology, â€Å"Active transport uses energy to move a solute â€Å"uphill† against its gradient, whereas in facilitated diffusion, a solute moves down its concentration gradient and no energy input is required. If an experiment was conducted where the conditions of transfer were manipulated by adding in larger membrane pores, increasing protein carriers, increasing pressure and adding higher levels of ATP for active transport the rates of transfer will increase providing an optimal level of reactions. E xperimental Methods and Materials In conducting this experiment the materials needed were a computer the PhysioEX 8. 0 C D and the Anatomy and Physiology Lab Manual because this was a computer simulated experiment. Activity One: Simple DiffusionTwo beakers were placed next to each other and joined by a membrane holder. Four membranes were used and each possessed a different molecular weight cut off (MWCO) consisting of 20, 50, 100, and 200 MWCO; and were tested using NaCl, Urea, Albumin, and Glucose solutions. First, the 20 MWCO membrane was placed in the membrane holder between the beakers and the first solute studied was NaCl. A 9mM concentrated solution was dispersed into the left beaker and the right beaker was filled with deionized water. This transfer was allowed 60 minutes.At the end of this time lapse the results were recorded (see result section of the report). The 20 MWCO membrane was removed and each beaker was flushed for the next run. A membrane with the 50 MWCO was pla ced between the beakers and the steps performed above were repeated using the 9 mM NaCl solution for 60 min. and then repeated again for the 100 and 200 MWCO, as described by the A & P Lab Manual by Marieb and Mitchell. The next solutions tested were Albumin, Urea, and Glucose. All were placed into the left beaker independently and the tests were run exactly like that for NaCl.Activity Two: Facilitated Diffusion In this experiment the set-up of the two beakers and membrane holder was used again. Only NaCl and Glucose solutes were used and membranes with 500, 700 and 900 glucose carrier proteins The 500 membrane was placed between the beakers and the glucose solution with a concentration of 2. 00mM was delivered to the left beaker. The right beaker was filled with deionized water. The timer was set for 60 minutes. When the time was up the data was recorded and the beakers were flushed to set up for the next run.The same steps were repeated using the 2. 00 mM glucose solution with the 700 and 900 carrier protein membranes, separately for 60 minutes. The last run of this transport mechanism was done by increasing the 2. 00mM to 8. 00mM glucose concentration. This experiment was done the same way as above for each of the 500, 700 and 900 carrier protein membranes for 60 min. respectively. Activity 3: Osmotic Pressure In this experiment pressure readers were added in order measure osmotic pressure change and were placed on top the two beakers.A 20 MWCO membrane was placed between the beakers and a NaCl concentration of 8mM was put into the left beaker. Deionized water was placed into the right beaker. Time was set at 60 minutes. The pressure steps were repeated with the 50, 100 and 200 MWCO membranes Activity 4: Active Transport This experiment resembled the osmosis experiment except that an ATP dispenser was substituted for the pressure meters on top of the beakers. In this experiment it was assumed that the left beaker was the inside of the cell and the right bea ker was the extracellular space.The membrane used had 500 glucose carrier proteins and 500 sodium-potassium pumps. Membrane was placed between the beakers and a NaCl concentration of 9. 00mM was delivered into the left beaker and a KCl concentration of 6mM was dispensed into the right beaker. The ATP was the changing variable in this experiment. 1mM of ATP was dispensed and transfer was observed for 60 min. It was observed when no ATPmM was applied and finally when 3mM ATP was applied. Results Activity 1: Simple Diffusion TABLE 1 Dialysis Results (average diffusion rate in mM/min) Solute| Membrane (MWCO)| 20| 50| 100| 200| NaCl| No diffusion| 0. 0150| 0. 0150| 0. 0150| Urea| No diffusion| No diffusion| 0. 0094| 0. 0094| Albumin| No diffusion| No diffusion| No diffusion| No diffusion | Glucose| No diffusion| No diffusion| No diffusion| 0. 0040| NaCl had no diffusion until the 50 MWCO was introduced and then it had a constant rate through the larger pored membranes. Urea diffused at 1 00 MWCO and up. Albumin had no diffusion through any of the membranes and Glucose diffused only through the 200 MWCO membrane. Activity 2: Facilitated Diffusion TABLE 2Facilitated Diffusion Results (glucose transport rate (mM/min) | Number of glucose carrier proteins| Glucose concentration(m/M)| 500| 700| 900| 2. 00| 0. 0008| 0. 0010| 0. 0012| 8. 00| 0. 0023| 0. 0031| 0. 0038| As the number of glucose carrier proteins increased so did the rate of transfer for both concentrations of glucose. The higher concentration of the 8. 00 m/M had a faster rate than that of the 2. 00 m/M glucose concentration Activity 3: Osmotic Pressure TABLE 3 Membrane (MWCO) | Solute| 20| 50| 100| 200| Na* Cl-| 272| 0| 0| 0| Albumin| 136| 136| 136| 136|Glucose| 136| 136| 136| 0| The osmotic pressure was highest and only occurred with the 20 MWCO membrane. Albumin had a constant pressure of 136 mm Hg with every membrane and Glucose had constant pressure of 136 mm Hg until it was relieved when the 200 MWCO mem brane was introduced. Activity 4: Active Transport Table 4 Run: 1 Solute| ATP| Start Conc. L| Start Conc. R| Pumps| Carriers| Rate| Na*| 1. 00| 9. 00| 0. 00| 500| ——–| 0. 0270| K*| 1. 00| 0. 00| 6. 00| 500| ——| 0. 0180| Glucose| ———| 0. 00| 0. 00| ——-| 500| 0. 0000| Run: 2 Solute| ATP | Start Conc. L| Start Conc.R| Pumps| Carriers| Rate| Na*| 0. 00| 9. 00| 0. 00| 500| ——–| 0. 0000| K*| 0. 00| 0. 00| 6. 00| 500| ——-| 0. 0000| Glucose| ———-| 0. 00| 0. 00| ——–| 500| 0. 0000| Run: 3 Solute| ATP | Start Conc. L| Start Conc. R| Pumps| Carriers| Rate| Na*| 3. 00| 9. 00| 0. 00| 500| —–| 0. 0050| K*| 3. 00| 0. 00| 6. 00| 500| —–| 0. 0033| Glucose| ———| 0. 00| 0. 00| ———| 500| 0. 0000| When 1 ATP was dispensed the Na and K transported at a higher rate than when 3 ATP was dispense d and there was no transport when ATP was absent. Discussion Activity 1: Simple DiffusionUpon observing the results for all of the solutes with the 20 MWCO membrane between the left beaker and the artificial external environment of deionized water in the right beaker no diffusion occurred, because the pores were not large enough for them to pass through. An observation that is important to note is that even the small ions of NaCl did not diffused here, so it is obvious that the other molecules would also not diffuse. At 50 MWCO the pores were just large enough for the dissociated NaCl ions to get through but the threshold stopped there because Urea, Albumin and Glucose molecules in the solute were too large.Observations of the diffusion of the solutes with the 100 MWCO membrane showed that all but albumin and Glucose passed, so urea size was now compatible for the size of this pore. Finally, when the 200 MWCO membrane was introduced everything except Glucose got through because it i s a very large molecule that cannot diffuse simply. It must be facilitated. Activity 2: Facilitated Diffusion In the facilitated diffusion of Glucose the parameters that were introduced were the number of carrier proteins available for transport in the membrane.According to the results, when there was a 2. 00mM concentration of Glucose in the left beaker there was evidence of diffusion based on the measured rate of diffusion in mM/min. As the number of carrier proteins increased by 200 between 500 and 900 the rate between 0 . 0008 to 0. 0012mM/ min also increased by 0. 0002 min into the beaker. When 8. 00mM of Glucose was placed in the left beaker with the same carrier protein membrane criteria of 500, 700, and 800 the rate increased. The rate was actually faster than that of the 2. 00 mM concentration.As the concentration of glucose raised the demand for the protein attachment increased so more carrier proteins got involved, while previously some were just hanging out because there was less glucose to transfer. Activity 3: Osmosis In this experiment the study was based on the transfer of water across a membrane. Osmosis of water tends to balance out concentrations, so it will flow to an area of higher solute concentration. Water flowing to a more concentrated solution will usually increase in volume but in this closed system for the experiment the focus was on the increase of pressure.The solutes were confined to their area by a semi-permeable membrane based on the pores of the membrane and the size of the molecules in the solute. With 8mM of NaCl with a 20 MWCO membrane the pressure reading was 272 mHg because the salt was not able to pass through the membrane, but the water diffused to the salt side so there was pressure causing and unequal balance, but with the membranes of 50, 100 and 200 MWCO there was no pressure because the membrane became permeable to the salt allowing an equilibrium between he beakers, therefore no pressure. In the case of Albumin, t he water diffused building up pressure until there was no more water left to diffuse so pressure remained constant at all MWCOs. The same occurred with Glucose until the membrane was replaced with the 200 MWCO membrane. Glucose was able to diffuse thus resulting in equilibrium in both beakers. Pressure will rise until equilibrium is obtained. Activity 4: Active TransportThe experiment showed that at 1 ATP the reaction took place at very slow rate and not completely. Without ATP the transfer didn’t take place at all. When 3 ATP’s were added transfer took place quickly and almost completely. The more ATP introduced to the cell, the faster and more complete the transport will occur which is very important for the transport of glucose since it is a substrate for the production of more ATP.